A:
Carrot Tissue Cultures
1. Define: a) cloning
b) totipotency
Production
of Carrot Callus:
2. a) From what part of the carrot is the explant taken?
b) Describe the callus that develops from this explant.
3. In this lab we use a box as our transfer case. List 2 methods by which
you try to
keep this area sterile.
4. In this lab everything must be kept sterile.
a) How is the equipment such as the scalpels and cork
borer sterilized?
b) How is the whole carrot sterilized?
c) How are your hands sterilized?
5. What agar is used with the carrot root explants?
APPEARANCE OF ROOT EXPLANTS (on petri dishes) 1-2 WEEKS LATER ________________________
Carrot Root
and Shoot Development:
6. What agar is used with the carrot callus?
7. This agar will stimulate the growth of what in the callus over the
next 6-8 weeks?
APPEARANCE OF CALLUS (in culture tubes) 1-2 WEEKS LATER ________________________________
B: African Violet Tissue Cultures
8. Define: a) in vivo
b) in vitro
9. Describe each of the following stages in the culturing of African Violets.
a) Stage I
b) Stage II
c) Stage III
d) Stage IV
10. Why are the following chemicals added to the agar?
a) adenine
b) auxins (IAA)
c) cytokinins (kinetin)
d) sucrose
Production
of African Violet Primordia & Plantlets:
11. To make African Violet explants:
a) Which leaves make the best explants?
b) Which part of the leaves make the best explants?
12. What is the purpose of the following chemicals used to prepare the
explant?
a) antioxidant
b) sodium hypochlorite
13. What agar is used with the African violet explants?
APPEARANCE OF AFRICAN VIOLET EXPLANTS (in square culture jars) 1-2 WEEKS
LATER __________
African Violet
Development into a Young Plant:
14. Over time tiny green bumps called primordial will develop, and grow
into tiny _______.
15. In stage III the larger plantlets should be transferred to another
container:
a) containing what type of agar?
b) to encourage growth of what?
APPEARANCE OF AFRICAN VIOLET PLANTLETS (in culture tubes) 1-2 WEEKS LATER
______________